Project description
The expertise of each partner is called upon for the development of specific aspects of the project, and for their integration. This includes:
-
Setting standard parameters of soil DNA extraction and selecting a best performing procedure
-
Develop sampling and storage procedures
-
Evaluate and compare DNA technologies applied to soil forensics
-
Delimit spatial and temporal boundaries of soil microbial profiling, and set up a soil profile database
-
To develop and implement bioinformatics and statistical tools required for soil microbial profiling
-
To evaluate and validate the protocols and the material, providing legal support to the approach
The methods developed are based on technologies and approaches already in use in forensic laboratories and are rapidly applicable.
-
Protocols for field sampling and sample collection, so that soil samples can be used for a diversity of analyses, including DNA-based analyses
-
Determination of DNA parameters and DNA extraction methods
-
Implementation of DNA technology for soil forensics
-
A conceptual framework for the use of microbial DNA in soil forensics
-
A framework for a prototype soil DNA geoforensics database
-
Bioinformatics and statistics tools for data analysis, providing the significance of the results acquired
-
Validation of the methods for use in forensic laboratories
